Publications

Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.
Phua SC, Chiba S, Suzuki M, Su E, Roberson EC, Pusapati GV, Setou M, Rohatgi R, Reiter JF, Ikegami K, Inoue T. 
Cell 168(1-2):264-279 (2017). PMID: 28086093.

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Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling.
Lebensohn AM, Dubey R, Neitzel LR, Tacchelly-Benites O, Yang E, Marceau CD, Davis EM, Patel BB, Bahrami-Nejad Z, Travaglini KJ, Ahmed Y, Lee E, Carette JE, Rohatgi R. 
eLife 5: e21459 (2016). PMID: 27996937.

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Cholesterol activates the G-protein coupled receptor Smoothened to promote Hedgehog signaling.
Luchetti G, Sircar R, Kong JH, Nachtergaele S, Sagner A, Byrne EF, Covey DF, Siebold C, Rohatgi R.
elife pii: e20304 (2016). PMID: 27705744.

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Chromatin-Remodeling Complex SWI/SNF Controls Multidrug Resistance by Transcriptionally Regulating the Drug Efflux Pump ABCB1.
Dubey R, Lebensohn AM, Bahrami-Nejad Z, Marceau C, Champion M, Gevaert O, Sikic BI, Carette JE, Rohatgi R.
Cancer Res 76(19):5810-5821 (2016). PMID: 27503929.

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In vivo formation of vacuolated multi-phase compartments lacking membranes.
Schmidt HB and Rohatgi R.
Cell Rep 16(5):1228-1236 (2016). PMID: 27452472.

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Structural basis of Smoothened regulation by its extracellular domains.
Byrne EFX, Sircar R, Miller PS, Hedger G, Luchetti G, Nachtergaele S, Tully MD, Mydock-McGrane L, Covey DF, Rambo RP, Sansom MSP, Newstead S, Rohatgi R, Siebold C.
Nature 535(7613):517-522 (2016). PMID: 27437577.

PubMED

An essential role for Grk2 in Hedgehog signaling downstream of Smoothened. 
Zhao Z, Lee RT, Pusapati GV, Iyu A, Rohatgi R, Ingham PW.
EMBO Rep 17(5):739-52 (2016). PMID: 27113758.

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Functional Divergence in the Role of N-Linked Glycosylation in Smoothened Signaling.
Marada S, Navarro G, Truong A, Stewart DP, Arensdorf AM, Nachtergaele S, Angelats E, Opferman JT, Rohatgi R, McCormick PJ, Ogden SK.
PLoS Genet 11(8):e1005473 (2015). PMID: 26291458.

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Notch activity modulates the responsiveness of neural progenitors to sonic hedgehog signaling.
Kong JH, Yang L, Dessaud E, Chuang K, Moore DM, Rohatgi R, Briscoe J, Novitch BG.
Dev Cell 33(4):373-87 (2015). PMID: 25936505.

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Rapid Screening of Gli2/3 Mutants Using the Flp-In System
Niewiadomski P and Rohatgi R.
Methods Mol Bio 1322:125-30 (2015). PMID: 26179044.

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Measuring Gli2 Phosphorylation by Selected Reaction Monitoring Mass Spectrometry.
Ahrends R, Niewiadomski P, Teruel MN, Rohatgi R.
Methods Mol Bio 1322:105-123 (2015). PMID: 26179041.

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Measuring Expression Levels of Endogenous Gli Genes by Immunoblotting and Real-Time PCR.
Niewiadomski P and Rohatgi R.
Methods Mol Bio 1322:91-92 (2015). PMID: 26179041.

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Location, location, and location: compartmentalization of Hedgehog signaling at primary cilia.
Pusapati GV and Rohatgi R.
EMBO J 33(17):1852-4 (2014). PMID: 25037564.

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Frontiers in hedgehog signal transduction.
Guerrero I and Rohatgi R.
Semin Cell Dev Biol 33:50-1 (2014). PMID: 24946961.

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A novel osteogenic oxysterol compound for therapeutic development to promote bone growth: activation hedgehog signaling and osteogenesis through smoothened binding.
Montgomery SR, Nargizyan T, Meliton V, Nachtergaele S, Rohatgi R, Stappenbeck F, Jung ME, Johnson JS, Aghdasi B, Tian H, Weintraub G, Inoue H, Atti E, Tetradis S, Pereira RC, Hokugo A, Alobaidaan R, Tan Y, Hahn TJ, Wang JC, Parhami F. 
Journal of Bone and Mineral Research 29(8):1872-85 (2014). PMID: 24591126.

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G-protein-coupled receptors, Hedgehog signaling and primary cilia. Seminars in Cell and Developmental Biology.  
Mukhopadhyay S and Rohatgi R. 
EPub prior to print (2014). PMID: 24845016.

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Tracking the subcellular fate of 20(S)-hydroxycholesterol with click chemistry reveals a transport pathway to the Golgi. 
Peyrot SM, Nachtergaele S, Luchetti G, Mydock-McGrane LK, Fujiwara H, Scherrer D, Jallouk A, Schlesinger PH, Ory DS, Covey DF, Rohatgi R. 
Journal of Biological Chemistry 289(16):11095-11110 (2014). PMID: 24596093.

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EFCAB7 and IQCE regulate Hedgehog signaling by tethering the EVC-EVC2 complex to the base of primary cilia. 
Pusapati GV, Hughes CE, Dorn KV, Zhang D, Sugianto P, Aravind L*, and Rohatgi R*. 
Developmental Cell 28(5):483-496 (2014). PMID: 24582806.

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Gli protein activity is controlled by multi-site phosphorylation in vertebrate Hedgehog signaling.
Niewiadomski P*, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel MN, Novitch BG, Rohatgi R*. 
Cell Reports 6(1):168-81 (2014). PMID: 24373970.

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Structure and function of the Smoothened extracellular domain in vertebrate Hedgehog signaling. 
Nachtergaele S, Whalen DM, Mydock LK, Zhao Z, Malinauskas T, Krishnan K, Ingham PW, Covey DF, Siebold C*, Rohatgi R*. 
Elife 2:e01340 (2013). PMID: 24171105.

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Chemically inducible diffusion trap at cilia reveals molecular sieve-like barrier.  
Lin YC, Niewiadomski P, Lin B, Nakamura H, Phua SC, Jiao J, Levchenko A, Inoue T, Rohatgi R, Inoue T. 
Nature Chemical Biology 9(7):437-443 (2013).  PMID: 23666116.

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Isolation and mutational analysis of circulating tumor cells from lung cancer patients with magnetic sifters and biochips. 
Earhart CM, Hughes CE, Gaster RS, Ooi CC, Wilson RJ, Zhou LY, Humke EW, Xu L, Wong DJ, Willingham SB, Schwartz EJ, Weissman IL, Jeffrey SS, Neal JW, Rohatgi R, Wakelee HA, Wang SX. 
Lab on a Chip. 14(1), 78-88 (2013). PMID: 23969419.

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Cancer risk after use of recombinant bone morphogenetic protein-2 for spinal arthrodesis. 
Carragee EJ, Chu G, Rohatgi R, Hurwitz EL, Weiner BK, Yoon ST, Comer G, Kopjar B. 
Journal of Bone and Joint Surgery 95(17), 1537-45 (2013). PMID: 24005193.

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Oxysterols are allosteric activators of the oncoprotein Smoothened. 
Nachtergaele S, Mydock LK, Krishnan K, Rammohan J, Schlesinger PH, 
Covey DF, Rohatgi R.
Nat Chem Biol 8(2):211-20 (2012). PMID: 22231273.

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A Smoothened-Evc2 complex transduces the Hedgehog signal at primary cilia. Dorn K, Hughes CE and Rohatgi R.  
Developmental Cell 23(4):823-35 (2012). PMID: 22981989.

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Singapore signalling: the 2012 hedgehog pathway cocktail. 
Briscoe J, Rohatgi R. 
EMBO Rep 13(7):580-3 (2012). PMID: 22688966.

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The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteins. 
Humke EW, Dorn KV, Milenkovic L, Scott MP, Rohatgi R. 
Genes Dev 24(7):670-82 (2010). PMID: 20360384.

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The ciliary membrane. 
Rohatgi R, Snell WJ. 
Curr Opin Cell Biol 22(4):541-6 (2010). PMID: 20399632.

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Hedgehog signal transduction by smoothened: pharmacological evidence for a two-step activation process. 
Rohatgi R, Milenkovic L, Corcoran RB, and Scott MP. 
Proceedings of the National Academy of Sciences USA 106(9):3196-201 (2009). PMID: 19218434.

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Lateral transport of Smoothened from the plasma membrane to the membrane of the cilium.
Milenkovic L, Scott MP, Rohatgi R. 
J Cell Biol. 2009; 187 (3): 365-74

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Arrestin Movement in Cilia. 
Rohatgi R, Scott MP. 
Science. 2008; 320 (5884): 1777-1781

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Patched1 regulates Hedgehog signaling at the primary cilium. 
Rohatgi R, Milenkovic, Scott MP. 
Science. 2007; 317 (5836): 372-376

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Patching the gaps in Hedgehog signaling. 
Rohatgi R, Scott MP. 
Nat Cell Bio. 2007; 9 (9): 1005-1009

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In vitro reconstitution of cdc42-mediated actin assembly using purified components. 
Ho HY, Rohatgi R, Lebensohn A, Kirschner MW. 
Methods in Enzymology. 2006: 406 174-190

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Loss-of-function Analysis of EphA Receptors in Retinotectal mapping. 
Feldheim DA, Nakamoto M, Osterfield M, Gale NW, DeChiara TM, Rohatgi R, Yancopoulos GD, Flanagan JG. 
Journal of Neuroscience. 2004; 24 (10): 2542-2550

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Toca-1 Mediates Cdc42- Dependent Actin Nucleation by Activating the N-WASP-WIP Complex. 
Ho HY, Rohatgi R, Lebensohn A, Ma L, Li L, Gygi SP, Kirschner MW. 
Cell. 2004; 118 (2): 203-216

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The Mechanism of Regulation of WAVE1-induced Actin Nucleation by Rac1 and Nck.
Eden S, Rohatgi R, Podtelejnikov AV, Mann M, and Kirschner MW. 
Nature. 2002; 418 (6899): 790-793

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CR16 Forms a Complex with N-WASP in Brain and is a Novel Member of a Conserved Proline-Rich Actin-Binding Protein Family. 
Ho HY, Rohatgi R, Ma L, and Kirschner MW. 
Proceedings of the National Academy of Sciences USA. 2001; 98 (20): 11306-11311

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Nck and Phosphatidylinositol 4,5 Bisphosphate Synergistically Activate Actin Polymerization Through the N-WASP-Arp2/3 Pathway. 
Rohatgi R, Nollau P, Ho HY, Kirschner MW, and Mayer BJ. 
Journal of Biological Chemistry. 2001; 276 (28): 26448-26452

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WIP Regulates N-WASP-Mediated Actin Polymerization and Filopodium Formation.
Martinez-Quiles N, Rohatgi R, Anton IM, Medina M, Saville SP, Miki H, Yamaguchi H, Takenawa T, Hartwig JH, Geha RS, Ramesh N. 
Nature Cell Biology. 2001; 3 (5): 484-491

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Mechanism of N-WASP Activation by CDC42 and Phosphatidylinositol 4, 5-Bisphosphate. 
Rohatgi R, Ho HY, Kirschner MW. 
Journal of Cell Biology. 2000; 150 (6): 1299-1310

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The Interaction Between N-WASP and the Arp2/3 Complex Links Cdc42-Dependent Signals to Actin Assembly. 
Rohatgi R, Ma L, Miki H, Lopez M, Kirchhausen T, Takenawa T, Kirschner MW. 
Cell. 1999; 97 (2): 221-231

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The Arp2/3 Complex Mediates Actin Polymerization Induced by the Small GTP-Binding Protein Cdc42. 
Ma L, Rohatgi R, Kirschner MW. 
Proceedings of the National Academy of Sciences USA. 1998; 95 (26): 15362-15367

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Kinetic and Mechanistic Analysis of Non-Enzymatic, Template-Directed Oligoribonucleotide Ligation. 
Rohatgi R, Bartel DP, Szostak JW. 
Journal of the American Chemical Society. 1996; 118 (14): 3332-3339

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Non-Enzymatic, Template-Directed Ligation of Oligoribonucleotides is Highly Regioselective for the Formation of 3'-5'-Phosphodiester Bonds
Rohatgi R, Bartel DP, Szostak JW. 
Journal of the American Chemical Society. 1996; 118 (14): 3340-3344

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