publications
2018
A single N-terminal phosphomimic disrupts TDP-43 polymerization, phase separation, and RNA splicing.
Wang A, Conicella AE, Schmidt HB, Martin EW, Rhoads SN, Reeb AN, Nourse A, Ramirez Montero D, Ryan VH, Rohatgi R, Shewmaker F, Naik MT, Mittag T, Ayala YM, Fawzi NL
EMBO J pii: e97452 (2018). PMID: 29438978.
PDF|PubMED
G protein-coupled receptors control the sensitivity of cells to the morphogen Sonic Hedgehog.
Pusapati GV, Kong JH, Patel BB, Gouti M, Sagner A, Sircar R, Luchetti G, Ingham PW, Briscoe J, Rohatgi R.
Sci Signal 11(516): eaa05749 (2018). PMID: 29438014.
PDF|PubMED
R-spondins can potentiate WNT signaling without LGRs
Lebensohn AM, Rohatgi R.
Elife pii: e33126 (2018). PMID: 29405118.
PDF|PubMED
CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity to the Morphogen Sonic Hedgehog.
Pusapati GV, Kong JH, Patel BB, Krishnan A, Sagner A, Kinnebrew M, Briscoe J, Aravind L, Rohatgi R.
Dev Cell 44(1):113-129.e8 (2018). PMID: 29290584.
PDF |PubMED
2017
Multiple ligand binding sites regulate the Hedgehog signal transducer Smoothened in vertebrates.
Byrne EF, Luchetti G, Rohatgi R, Siebold C.
Curr Opin Cell Biol 51:81-88 (2017). PMID: 29268141.
PDF |PubMED
Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.
Phua SC, Chiba S, Suzuki M, Su E, Roberson EC, Pusapati GV, Setou M, Rohatgi R, Reiter JF, Ikegami K, Inoue T.
Cell 168(1-2):264-279 (2017). PMID: 28086093.
PDF |PubMED
2016
Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling.
Lebensohn AM, Dubey R, Neitzel LR, Tacchelly-Benites O, Yang E, Marceau CD, Davis EM, Patel BB, Bahrami-Nejad Z, Travaglini KJ, Ahmed Y, Lee E, Carette JE, Rohatgi R.
eLife 5: e21459 (2016). PMID: 27996937.
PDF |PubMED
Cholesterol activates the G-protein coupled receptor Smoothened to promote Hedgehog signaling.
Luchetti G, Sircar R, Kong JH, Nachtergaele S, Sagner A, Byrne EF, Covey DF, Siebold C, Rohatgi R.
elife pii: e20304 (2016). PMID: 27705744.
PDF |PubMED
Chromatin-Remodeling Complex SWI/SNF Controls Multidrug Resistance by Transcriptionally Regulating the Drug Efflux Pump ABCB1.
Dubey R, Lebensohn AM, Bahrami-Nejad Z, Marceau C, Champion M, Gevaert O, Sikic BI, Carette JE, Rohatgi R.
Cancer Res 76(19):5810-5821 (2016). PMID: 27503929.
PDF |PubMED
In vivo formation of vacuolated multi-phase compartments lacking membranes.
Schmidt HB and Rohatgi R.
Cell Rep 16(5):1228-1236 (2016). PMID: 27452472.
PDF |PubMED
Structural basis of Smoothened regulation by its extracellular domains.
Byrne EFX, Sircar R, Miller PS, Hedger G, Luchetti G, Nachtergaele S, Tully MD, Mydock-McGrane L, Covey DF, Rambo RP, Sansom MSP, Newstead S, Rohatgi R, Siebold C.
Nature 535(7613):517-522 (2016). PMID: 27437577.
PDF |PubMED
An essential role for Grk2 in Hedgehog signaling downstream of Smoothened.
Zhao Z, Lee RT, Pusapati GV, Iyu A, Rohatgi R, Ingham PW.
EMBO Rep 17(5):739-52 (2016). PMID: 27113758.
PDF |PubMED
2015
Functional Divergence in the Role of N-Linked Glycosylation in Smoothened Signaling.
Marada S, Navarro G, Truong A, Stewart DP, Arensdorf AM, Nachtergaele S, Angelats E, Opferman JT, Rohatgi R, McCormick PJ, Ogden SK.
PLoS Genet 11(8):e1005473 (2015). PMID: 26291458.
PDF |PubMED
Notch activity modulates the responsiveness of neural progenitors to sonic hedgehog signaling.
Kong JH, Yang L, Dessaud E, Chuang K, Moore DM, Rohatgi R, Briscoe J, Novitch BG.
Dev Cell 33(4):373-87 (2015). PMID: 25936505.
PDF |PubMED
Rapid Screening of Gli2/3 Mutants Using the Flp-In System
Niewiadomski P and Rohatgi R.
Methods Mol Bio 1322:125-30 (2015). PMID: 26179044.
PDF |PubMED
Measuring Gli2 Phosphorylation by Selected Reaction Monitoring Mass Spectrometry.
Ahrends R, Niewiadomski P, Teruel MN, Rohatgi R.
Methods Mol Bio 1322:105-123 (2015). PMID: 26179041.
PDF |PubMED
Measuring Expression Levels of Endogenous Gli Genes by Immunoblotting and Real-Time PCR.
Niewiadomski P and Rohatgi R.
Methods Mol Bio 1322:91-92 (2015). PMID: 26179041.
PDF |PubMED
2014
Location, location, and location: compartmentalization of Hedgehog signaling at primary cilia.
Pusapati GV and Rohatgi R.
EMBO J 33(17):1852-4 (2014). PMID: 25037564.
PDF |PubMED
Frontiers in hedgehog signal transduction.
Guerrero I and Rohatgi R.
Semin Cell Dev Biol 33:50-1 (2014). PMID: 24946961.
PDF |PubMED
A novel osteogenic oxysterol compound for therapeutic development to promote bone growth: activation hedgehog signaling and osteogenesis through smoothened binding.
Montgomery SR, Nargizyan T, Meliton V, Nachtergaele S, Rohatgi R, Stappenbeck F, Jung ME, Johnson JS, Aghdasi B, Tian H, Weintraub G, Inoue H, Atti E, Tetradis S, Pereira RC, Hokugo A, Alobaidaan R, Tan Y, Hahn TJ, Wang JC, Parhami F.
Journal of Bone and Mineral Research 29(8):1872-85 (2014). PMID: 24591126.
PDF |PubMED
G-protein-coupled receptors, Hedgehog signaling and primary cilia. Seminars in Cell and Developmental Biology.
Mukhopadhyay S and Rohatgi R.
EPub prior to print (2014). PMID: 24845016.
PDF |PubMED
Tracking the subcellular fate of 20(S)-hydroxycholesterol with click chemistry reveals a transport pathway to the Golgi.
Peyrot SM, Nachtergaele S, Luchetti G, Mydock-McGrane LK, Fujiwara H, Scherrer D, Jallouk A, Schlesinger PH, Ory DS, Covey DF, Rohatgi R.
Journal of Biological Chemistry 289(16):11095-11110 (2014). PMID: 24596093.
PDF |PubMED
EFCAB7 and IQCE regulate Hedgehog signaling by tethering the EVC-EVC2 complex to the base of primary cilia.
Pusapati GV, Hughes CE, Dorn KV, Zhang D, Sugianto P, Aravind L*, and Rohatgi R*.
Developmental Cell 28(5):483-496 (2014). PMID: 24582806.
PDF |PubMED
Gli protein activity is controlled by multi-site phosphorylation in vertebrate Hedgehog signaling.
Niewiadomski P*, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel MN, Novitch BG, Rohatgi R*.
Cell Reports 6(1):168-81 (2014). PMID: 24373970.
PDF |PubMED
2013
Structure and function of the Smoothened extracellular domain in vertebrate Hedgehog signaling.
Nachtergaele S, Whalen DM, Mydock LK, Zhao Z, Malinauskas T, Krishnan K, Ingham PW, Covey DF, Siebold C*, Rohatgi R*.
Elife 2:e01340 (2013). PMID: 24171105.
PDF |PubMED
Chemically inducible diffusion trap at cilia reveals molecular sieve-like barrier.
Lin YC, Niewiadomski P, Lin B, Nakamura H, Phua SC, Jiao J, Levchenko A, Inoue T, Rohatgi R, Inoue T.
Nature Chemical Biology 9(7):437-443 (2013). PMID: 23666116.
PDF |PubMED
Isolation and mutational analysis of circulating tumor cells from lung cancer patients with magnetic sifters and biochips.
Earhart CM, Hughes CE, Gaster RS, Ooi CC, Wilson RJ, Zhou LY, Humke EW, Xu L, Wong DJ, Willingham SB, Schwartz EJ, Weissman IL, Jeffrey SS, Neal JW, Rohatgi R, Wakelee HA, Wang SX.
Lab on a Chip. 14(1), 78-88 (2013). PMID: 23969419.
PDF |PubMED
Cancer risk after use of recombinant bone morphogenetic protein-2 for spinal arthrodesis.
Carragee EJ, Chu G, Rohatgi R, Hurwitz EL, Weiner BK, Yoon ST, Comer G, Kopjar B.
Journal of Bone and Joint Surgery 95(17), 1537-45 (2013). PMID: 24005193.
PDF |PubMED
2012
Oxysterols are allosteric activators of the oncoprotein Smoothened.
Nachtergaele S, Mydock LK, Krishnan K, Rammohan J, Schlesinger PH,
Covey DF, Rohatgi R.
Nat Chem Biol 8(2):211-20 (2012). PMID: 22231273.
PDF |PubMED
A Smoothened-Evc2 complex transduces the Hedgehog signal at primary cilia. Dorn K, Hughes CE and Rohatgi R.
Developmental Cell 23(4):823-35 (2012). PMID: 22981989.
PDF |PubMED
Singapore signalling: the 2012 hedgehog pathway cocktail.
Briscoe J, Rohatgi R.
EMBO Rep 13(7):580-3 (2012). PMID: 22688966.
PDF |PubMED
2010
The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteins.
Humke EW, Dorn KV, Milenkovic L, Scott MP, Rohatgi R.
Genes Dev 24(7):670-82 (2010). PMID: 20360384.
PDF |PubMED
The ciliary membrane.
Rohatgi R, Snell WJ.
Curr Opin Cell Biol 22(4):541-6 (2010). PMID: 20399632.
PDF |PubMED
2009
Hedgehog signal transduction by smoothened: pharmacological evidence for a two-step activation process.
Rohatgi R, Milenkovic L, Corcoran RB, and Scott MP.
Proceedings of the National Academy of Sciences USA 106(9):3196-201 (2009). PMID: 19218434.
PDF |PubMED
Lateral transport of Smoothened from the plasma membrane to the membrane of the cilium.
Milenkovic L, Scott MP, Rohatgi R.
J Cell Biol. 2009; 187 (3): 365-74
PDF |PubMED
2008
Arrestin Movement in Cilia.
Rohatgi R, Scott MP.
Science. 2008; 320 (5884): 1777-1781
PDF |PubMED
2007
Patched1 regulates Hedgehog signaling at the primary cilium.
Rohatgi R, Milenkovic, Scott MP.
Science. 2007; 317 (5836): 372-376
PDF |PubMED
Patching the gaps in Hedgehog signaling.
Rohatgi R, Scott MP.
Nat Cell Bio. 2007; 9 (9): 1005-1009
PDF |PubMED
2006
In vitro reconstitution of cdc42-mediated actin assembly using purified components.
Ho HY, Rohatgi R, Lebensohn A, Kirschner MW.
Methods in Enzymology. 2006: 406 174-190
PDF |PubMED
2004
Loss-of-function Analysis of EphA Receptors in Retinotectal mapping.
Feldheim DA, Nakamoto M, Osterfield M, Gale NW, DeChiara TM, Rohatgi R, Yancopoulos GD, Flanagan JG.
Journal of Neuroscience. 2004; 24 (10): 2542-2550
PDF |PubMED
Toca-1 Mediates Cdc42- Dependent Actin Nucleation by Activating the N-WASP-WIP Complex.
Ho HY, Rohatgi R, Lebensohn A, Ma L, Li L, Gygi SP, Kirschner MW.
Cell. 2004; 118 (2): 203-216
PDF |PubMED
2002
The Mechanism of Regulation of WAVE1-induced Actin Nucleation by Rac1 and Nck.
Eden S, Rohatgi R, Podtelejnikov AV, Mann M, and Kirschner MW.
Nature. 2002; 418 (6899): 790-793
PDF |PubMED
2001
CR16 Forms a Complex with N-WASP in Brain and is a Novel Member of a Conserved Proline-Rich Actin-Binding Protein Family.
Ho HY, Rohatgi R, Ma L, and Kirschner MW.
Proceedings of the National Academy of Sciences USA. 2001; 98 (20): 11306-11311
PDF |PubMED
Nck and Phosphatidylinositol 4,5 Bisphosphate Synergistically Activate Actin Polymerization Through the N-WASP-Arp2/3 Pathway.
Rohatgi R, Nollau P, Ho HY, Kirschner MW, and Mayer BJ.
Journal of Biological Chemistry. 2001; 276 (28): 26448-26452
PDF |PubMED
WIP Regulates N-WASP-Mediated Actin Polymerization and Filopodium Formation.
Martinez-Quiles N, Rohatgi R, Anton IM, Medina M, Saville SP, Miki H, Yamaguchi H, Takenawa T, Hartwig JH, Geha RS, Ramesh N.
Nature Cell Biology. 2001; 3 (5): 484-491
PDF |PubMED
2000
Mechanism of N-WASP Activation by CDC42 and Phosphatidylinositol 4, 5-Bisphosphate.
Rohatgi R, Ho HY, Kirschner MW.
Journal of Cell Biology. 2000; 150 (6): 1299-1310
PDF |PubMED
1999
The Interaction Between N-WASP and the Arp2/3 Complex Links Cdc42-Dependent Signals to Actin Assembly.
Rohatgi R, Ma L, Miki H, Lopez M, Kirchhausen T, Takenawa T, Kirschner MW.
Cell. 1999; 97 (2): 221-231
PDF |PubMED
1998
The Arp2/3 Complex Mediates Actin Polymerization Induced by the Small GTP-Binding Protein Cdc42.
Ma L, Rohatgi R, Kirschner MW.
Proceedings of the National Academy of Sciences USA. 1998; 95 (26): 15362-15367
PDF |PubMED
1996
Kinetic and Mechanistic Analysis of Non-Enzymatic, Template-Directed Oligoribonucleotide Ligation.
Rohatgi R, Bartel DP, Szostak JW.
Journal of the American Chemical Society. 1996; 118 (14): 3332-3339
PDF |PubMED
Non-Enzymatic, Template-Directed Ligation of Oligoribonucleotides is Highly Regioselective for the Formation of 3'-5'-Phosphodiester Bonds
Rohatgi R, Bartel DP, Szostak JW.
Journal of the American Chemical Society. 1996; 118 (14): 3340-3344
PDF |PubMED