bold — Rohatgi Lab member
* — equal contribution
^ — corresponding author

PREPRINTS

Plasma membrane accessible cholesterol is regulated by ACC1 and lipid droplets.

Wijesinghe KM, Kim CW, Schad EO, Li S, Chen S, Takeshima E, Khandwala CB, Tillo D, Lebensohn AM, Olzmann JA, Rohatgi R, Kinnebrew M.
bioRxiv (2025) doi: https://doi.org/10.1101/2025.08.21.671640

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cited by 2 [Google Scholar]

PUBLICATIONS

2026

Mutational scanning reveals substrate-assisted autoregulation of the WNT destruction complex.

Padmanarayana M*, Sakalas S, Sarkar P, Ma M, Garvin ER, Lee E, Corsello SM, Guettler S, Pusapati GV*^, Rohatgi R^. (*equal contribution; ^corresponding author)
Nature Genetics 58(7):1672-1686 (2026). PMID: 42393221

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Design principles of a membrane-spanning ubiquitin ligase.

Williams C*, Nocka LM*, Hedger G, Parashara P, Pardon E, Latorraca NR, Pusapati GV, Sarkar P, Lartey D, Gao L, Milenkovic L, Chalk R, Steyaert J, Marqusee S, Carrique L, Bazan JF, Rouse SL, Kong JH, Siebold C^, Rohatgi R^. (*equal contribution; ^corresponding author)
Molecular Cell 86(11):2207-2222 (2026). PMID: 42190653

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cited by 4 [Google Scholar]

Regulation of EIF5A and hypusination by p53 determines colorectal cancer cell fitness.

Gobert AP^, Hawkins CV, Asim M, Barry DP, Delgado AG, Tyree RN, Carson KS, Kahlon NPS, Ciampa MV, Rose KL, Patel P, Pusapati GV, Rohatgi R, Yu W, Zhao S, Coburn LA, Blanca Piazuelo M, Wilson KT^. (^corresponding author)
Cancer Letters 656:218682 (2026). PMID: 42297232.

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Multiple modes of cholesterol translocation in the human Smoothened receptor.

Bansal P, Kinnebrew M, Rohatgi R, Shukla D^. (^corresponding author)
eLife 14:RP108030 (2026). PMID: 41811180

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cited by 1 [Google Scholar]

Expanding roles of N-glycosylation in the endoplasmic reticulum.

Ma M^, Rohatgi R^. (^corresponding author)
Trends in Cell Biology https://doi.org/10.1016/j.tcb.2025.12.001 Jan 14 2026.

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cited by 1 [Google Scholar]

Structural basis of regulated N-glycosylation at the secretory translocon.

Yamsek M*, Ma M *, Jha R *, Wan Y, Li Q, Zhong F, DeLong K, Ji Z, Rohatgi R^, Keenan R^. (*equal contribution; ^corresponding author)
Nature 649:777–784 (2026). PMID: 41261126

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cited by 6 [Google Scholar]

2025

The TRIP12 E3 ligase induces SWI/SNF component BRG1-β-catenin interaction to promote Wnt signaling.

Kassel S, Yuan K, Bunnag N, Neitzel LR, Lu W, Schwarzkopf A, Maines B, Loberg MA, Xu G, Adams A, McCray AD, Cho A, Rockouski M, Orton G, Goldsmith L, Aronno MMA, Spencer ZT, Khan OM, Ye F, Williams C, Lebensohn AM, Rohatgi R, Wang X, Weiss VL, Hong CC, Kettenbach AN, Robbins DJ, Ahmed Y^, Lee E^. (^corresponding author).
Nature Communications 16:5248 (2025). PMID: 40473626

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cited by 6 [Google Scholar]

Direct ionic stress sensing and mitigation by the transcription factor NFAT5

Khandwala CB*, Sarkar P*, Schmidt HB, Ma M, Pusapati GV, Lamoliatte F, Kinnebrew M, Patel BB, Tillo D, Lebensohn AM, Rohatgi R^. (*equal contribution, ^corresponding author).
Science Advances 11: eadu3194 (2025). PMID: 39970224

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cited by 9 [Google Scholar]

Serotonin signaling at cilia synapses.

DeLong K, Sheu SH^. (^corresponding author).
Current Opinion in Neurobiology 92:102994 (2025). PMID: 40081222.

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cited by 7 [Google Scholar]

2024

Regulated N-glycosylation controls chaperone function and receptor trafficking.

Ma M, Dubey R, Jen A, Pusapati GV, Singal B, Shishkova E, Overmyer KA, Cormier-Daire V, Fedry J, Aravind L, Coon JJ, Rohatgi R^. (^corresponding author).
Science 386: 667-672(2024). PMID: 39509507

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cited by 28 [Google Scholar]

The exocyst complex and intracellular vesicles mediate soluble protein trafficking to the primary cilium.

Niedziółka SM, Datta S, Uśpieński T, Baran B, Skarżyńska W, Humke EW, Rohatgi R, Niewiadomski P^. ( ^corresponding author).
Communications Biology 7(1):213 (2024) PMID: 38378792

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cited by 14 [Google Scholar]

A cholesterol-binding bacterial toxin provides a strategy for identifying a specific Scap inhibitor that blocks lipid synthesis in animal cells.

Xu S, Smothers JC, Rye D, Endapally S, Chen H, Li S, Liang G, Kinnebrew M, Rohatgi R, Posner BA, Radhakrishnan A^. (^corresponding author) Proceedings of the National Academy of Sciences USA 121(7):e2318024121 (2024) PMID: 38330014

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cited by 16 [Google Scholar]

2023

The USP46 complex deubiquitylates LRP6 to promote Wnt/β-catenin signaling.

Ng VH*, Spencer Z*, Neitzel LR, Nayak A, Loberg MA, Shen C, Kassel SN, Kroh HK, An Z, Anthony CC, Bryant JM, Lawson A, Goldsmith L, Benchabane H, Hansen AG, Li J, D'Souza S, Lebensohn AM, Rohatgi R, Weiss WA, Weiss VL, Williams C, Hong CC, Robbins DJ, Ahmed Y^, Lee E.^ (*equal contribution; ^corresponding author) Nature Communications 14(1):6173. (2023) PMID: 37798301

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cited by 27 [Google Scholar]

The USP46 deubiquitylase complex increases Wingless/Wnt signaling strength by stabilizing Arrow/LRP6.

Spencer ZT*, Ng VH*, Benchabane H*, Siddiqui GS, Duwadi D, Maines B, Bryant JM, Schwarzkopf A, Yuan K, Kassel SN, Mishra A, Pimentel A, Lebensohn AM, Rohatgi R, Gerber SA, Robbins DJ, Lee E^, Ahmed Y^. (*equal contribution; ^corresponding author)
Nature Communications 14(1):6174. (2023) PMID: 37798281

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cited by 11 [Google Scholar]

The energetics and ion coupling of cholesterol transport through Patched1.

Ansell TB, Corey RA, Viti LV, Kinnebrew M, Rohatgi R, Siebold C, Sansom MSP^. (^corresponding author) Science Advances 9(34):eadh1609 (2023) PMID: 37611095

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cited by 11 [Google Scholar]

The Inseparable Relationship Between Cholesterol and Hedgehog Signaling.

Siebold C^ and Rohatgi R^ (^corresponding author)
Annual Review of Biochemistry 92:273-298 (2023) PMID: 37001135

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cited by 32 [Google Scholar]

2022

Oxaliplatin disrupts nucleolar function through biophysical disintegration.

Schmidt HB, Jaafar ZA, Wulff BE, Rodencal JJ, Hong K, Aziz-Zanjani MO, Jackson PK, Leonetti MD, Dixon SJ, Rohatgi R^, Brandman^ (^corresponding author) Cell Reports 41(6):111629. (2022). PMID: 36351392

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cited by 58 [Google Scholar]

Receptor control by membrane-tethered ubiquitin ligases in development and tissue homeostasis.

Lebensohn AM^, Bazan JF^, Rohatgi R^. (^corresponding author)
Current Topics in Developmental Biology 150:25-89 (2022). PMID: 35817504

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cited by 18 [Google Scholar]

Patched 1 regulates Smoothened by controlling sterol binding to its extracellular cysteine-rich domain.

Kinnebrew M, Woolley RE, Ansell TB, Byrne EFX, Frigui S, Luchetti G, Sircar R, Nachtergaele S, Mydock-McGrane L, Krishnan K, Newstead S, Sansom MSP, Covey DF, Siebold C^, Rohatgi R^. (^corresponding author) Science Advances 8(22):eabm5563 (2022). PMID: 35658032

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cited by 50 [Google Scholar]

Measuring and Manipulating Membrane Cholesterol for the Study of Hedgehog Signaling.

Kinnebrew M*, Johnson KA*, Radhakrishnan A^, Rohatgi R^. (*equal contribution; ^corresponding author)
Methods in Molecular Biology 2374:73-87. (2022) PMID: 34562244

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cited by 1 [Google Scholar]

2021

Hedgehog-Interacting Protein is a multimodal antagonist of Hedgehog signalling.

Griffiths SC*, Schwab RA*, El Omari K, Bishop B, Iverson EJ, Malinauskas T, Dubey R, Qian M, Covey DF, Gilbert RJC, Rohatgi R, Siebold C^. (*equal contribution; ^corresponding author)
Nature Communications 12(1):7171. (2021). PMID: 34887403

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cited by 41 [Google Scholar]

Patched 1 reduces the accessibility of cholesterol in the outer leaflet of membranes.

Kinnebrew M, Luchetti G, Sircar R, Frigui S, Viti LV, Naito T, Beckert F, Saheki Y, Siebold C, Radhakrishnan A, Rohatgi R^. (^corresponding author) eLife 10:e70504 (2021) PMID: 34698632

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cited by 68 [Google Scholar]

Gene-teratogen interactions influence the penetrance of birth defects by altering Hedgehog signaling strength.

Kong JH, Young CB, Pusapati GV, Espinoza FH, Patel CB, Beckert F, Ho S, Patel BP, Gabriel GC, Aravind L, Bazan JF, Gunn TM^, Lo CW^, and Rohatgi R^. (^corresponding author) Development 148 (19):dev199867 (2021). PMID: 34486668

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cited by 15 [Google Scholar]

Human-chimpanzee fused cells reveal cis-regulatory divergence underlying skeletal evolution.

Gokhman D^, Agoglia RM, Kinnebrew M, Gordon W, Sun D, Bajpai VK, Naqvi S, Chen C, Chan A, Chen C, Petrov DA, Ahituv N, Zhang H, Mishina Y, Wysocka J, Rohatgi R, and Fraser HB^. (^corresponding author)
Nature Genetics 53(4):467-476. (2021). PMID: 33731941

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cited by 88 [Google Scholar]

Bile acid biosynthesis in Smith-Lemli-Opitz syndrome bypassing cholesterol: Potential importance of pathway intermediates.

Abdel-Khalik J, Hearn T, Dickson AL, Crick PJ, Yutuc E, Austin-Muttitt K, Bigger BW, Morris AA, Shackleton CH, Clayton PT, Iida T, Sircar R, Rohatgi R, Marschall HU, Sjövall J, Björkhem I, Mullins JGL, Griffiths WJ^, and Wang Y^. (^corresponding author)
The Journal of Steroid Biochemistry and Molecular Biology 206:105794.(2021). PMID: 33246156

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cited by 31 [Google Scholar]

2020

Mutations in GRK2 cause Jeune syndrome by impairing Hedgehog and canonical Wnt signaling.

Bosakova M, Abraham SP, Nita A, Hruba E, Buchtova M, Taylor SP, Duran I, Martin J, Svozilova K, Barta T, Varecha M, Balek L, Kohoutek J, Radaszkiewicz T, Pusapati GV, Bryja V, Rush ET, Thiffault I, Nickerson DA, Bamshad MJ; University of Washington Center for Mendelian Genomics, Rohatgi R, Cohn DH, Krakow D^, and Krejci P^. (^corresponding author)
EMBO Molecular Medicine 12(11):e11739 (2020). PMID: 33200460

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cited by 33 [Google Scholar]

Cholesterol access in cellular membranes controls Hedgehog signaling.

Radhakrishnan A*^, Rohatgi R*^, and Siebold C*^. (*equal contribution; ^corresponding author)
Nature Chemical Biology 16(12):1303-1313 (2020). PMID: 33199907

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cited by 169 [Google Scholar]

A Membrane-Tethered Ubiquitination Pathway Regulates Hedgehog Signaling and Heart Development.

Kong JH*, Young CB*, Pusapati GV*, Patel CB, Ho S, Krishnan A, Lin JI, Devine W, Moreau de Bellaing A, Athni TS, Aravind L, Gunn TM^, Lo CW^, and Rohatgi R^. (*equal contribution; ^corresponding author)
Developmental Cell 55(4):432-449 (2020). PMID: 32966817

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cited by 58 [Google Scholar]

Flow Homogenization Enables a Massively Parallel Fluidic Design for High-throughput and Multiplexed Cell Isolation.

Ooi C^, Earhart CM, Hughes CE, Lee JR, Wong DJ, Wilson RJ, Rohatgi R, and Wang SX^. (^corresponding author)
Advanced Materials Technologies 5(5):1900960 (2020). PMID: 33072854

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cited by 1 [Google Scholar]

R-spondins engage heparin sulfate proteoglycans to potentiate WNT signaling.

Dubey R*, Kerkhof PV, Jordens I, Malinauskas T, Pusapati GV, McKenna JK, Li D, Carette JE, Ho M, Siebold C, Maurice M, Lebensohn AM*^, and Rohatgi R^. (*equal contribution; ^corresponding author)
eLife 9:e54469 (2020). PMID: 32432544

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cited by 62 [Google Scholar]

High-throughput Flow Cytometry Assay to Investigate TDP43 Splicing Function.

Schmidt HB^ and Rohatgi R. (^corresponding author)
Bio-protocol 10(8):e3594. (2020). PMID: 33659560

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cited by 6 [Google Scholar]

TDP-43 α-helical structure tunes liquid–liquid phase separation and function.

Conicella A*E, Dignon GL*, Zerze GH, Schmidt HB, Alexandra MD, Kim YC, Rohatgi R, Ayala YM, Mittal J^, and Fawzi NL^. (*equal contribution; ^corresponding author)
Proceedings of the National Academy of Sciences USA 117(11):5883-2894. (2020). PMID: 32132204

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cited by 437 [Google Scholar]

Lipid droplets can promote drug accumulation and activation.

Dubey R, Stivala CE, Nguyen HQ, Goo YH, Paul A, Carette JE, Trost BM, and Rohatgi R^.(^corresponding author)
Nature Chemical Biology 16(2):206-213 (2020) PMID: 31932720

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cited by 71 [Google Scholar]

2019

Cholesterol accessibility at the ciliary membrane controls hedgehog signaling.

Kinnebrew M*, Iverson EJ*, Patel BB, Pusapati GV, Kong JH, Johnson KA, Luchetti G, Eckert KM, McDonald JG, Covey DF, Siebold C, Radhakrishnan A^, and Rohatgi R^. (*equal contribution; ^corresponding author)
eLife 8:e50051 (2019). PMID: 31657721

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cited by 158 [Google Scholar]

Phase separation-deficient TDP43 remains functional in splicing.

Schmidt HB^, Barreau A, and Rohatgi R^. (^corresponding author)
Nature Communications 10(1):4890 (2019). PMID: 31653829

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cited by 208 [Google Scholar]

The morphogen Sonic Hedgehog inhibits its receptor Patched by a pincer grasp mechanism.

Rudolf AF*, Kinnebrew M*, Kowatsch C*, Ansell TB*, El Omari K*, Bishop B, Pardon E, Shwab RA, Malinauskas T, Qian M, Duman R, Covey DF, Steyaert J, Wagner A, Sansom MSP, Rohatgi R^, and Siebold C^. (*equal contribution; ^corresponding author)
Nature Chemical Biology 15(10):975-982. (2019). PMID: 31548691.

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cited by 89 [Google Scholar]

Structures of vertebrate Patched and Smoothened reveal intimate links between cholesterol and Hedgehog signalling.

Kowatsch C*, Woolley RE*, Kinnebrew M, Rohatgi R^, and Siebold C^. (*equal contribution; ^corresponding author)
Current Opinion in Structural Biology 57:204-214. (2019). PMID: 31247512

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cited by 69 [Google Scholar]

Biochemical mechanisms of vertebrate Hedgehog signaling.

Kong JH, Siebold C^, and Rohatgi R^. (^corresponding author)
Development 146(10):dev166892 (2019). PMID: 31092502.

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cited by 286 [Google Scholar]

Discovery of gene regulatory elements through a new bioinformatics analysis of haploid genetic screens.

Patel BB*, Lebensohn AM*^, Pusapati GV, Carette JE, Salzman J^, and Rohatgi R^. (*equal contribution; ^corresponding author)
PLoS One 14(1):e0198463 (2019). PMID: 30695034

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Cholesterol interaction sites on the transmembrane domain of the Hedgehog signal transducer and class F G protein-coupled receptor Smoothened.

Hedger G, Koldsø H, Chavent M, Siebold C, Rohatgi R, and Sansom MSP^. (^corresponding author)
Structure 27(3):549-559.e2 (2019). PMID: 30595453

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cited by 105 [Google Scholar]

2018

Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling.

Hong SR, Wang CL, Huang YS, Chang YC, Pusapati GV, Lin CY, Hsu N, Cheng HC, Chiang YC, Huang WE, Shaner NC, Rohatgi R, Inoue T^, and Lin YC^. (^corresponding author)
Nature Communications 9(1):1732 (2018). PMID: 29712905

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cited by 92 [Google Scholar]

A single N-terminal phosphomimic disrupts TDP-43 polymerization, phase separation, and RNA splicing.

Wang A*, Conicella AE*, Schmidt HB, Martin EW, Rhoads SN, Reeb AN, Nourse A, Ramirez Montero D, Ryan VH, Rohatgi R, Shewmaker F, Naik MT, Mittag T, Ayala YM, and Fawzi NL^. (*equal contribution; ^corresponding author)
The EMBO Journal 37(5): e97452 (2018). PMID: 29438978.

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cited by 525 [Google Scholar]

G-protein coupled receptors control the sensitivity of cells to the morphogen Sonic Hedgehog.

Pusapati GV*, Kong JH*, Patel BB, Gouti M, Sagner A, Sircar R, Luchetti G, Ingham PW, Briscoe J, and Rohatgi R^. (*equal contribution; ^corresponding author)
Science Signaling 11(516):eaa05749 (2018). PMID: 29438014.

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cited by 82 [Google Scholar]

R-spondins can potentiate WNT signaling without LGRs.

Lebensohn AM^ and Rohatgi R^. (^corresponding author)
eLife 7:e33126 (2018). PMID: 29405118.

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cited by 168 [Google Scholar]

CRISPR screens uncover genes that regulate target cell sensitivity to the morphogen Sonic Hedgehog.

Pusapati GV*^, Kong JH*, Patel BB*, Krishnan A, Sagner A, Kinnebrew M, Briscoe J, Aravind L, and Rohatgi^. (*equal contribution; ^corresponding author)
Developmental Cell 44(1):113-129 (2018). PMID: 29290584.

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cited by 174 [Google Scholar]

Multiple ligand binding sites regulate the Hedgehog signal transducer Smoothened in vertebrates.

Byrne EF, Luchetti G, Rohatgi R^, and Siebold C^. (^corresponding author)
Current Opinion in Cell Biology 51:81-88 (2018). PMID: 29268141.

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cited by 80 [Google Scholar]

2017

Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.

Phua SC^, Chiba S, Suzuki M, Su E, Roberson EC, Pusapati GV, Setou M, Rohatgi R, Reiter JF, Ikegami K^, and Inoue T^. (^corresponding author)
Cell 168(1-2):264-279 (2017). PMID: 28086093.

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cited by 419 [Google Scholar]

2016

Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling.

Lebensohn AM, Dubey R, Neitzel LR, Tacchelly-Benites O, Yang E, Marceau CD, Davis EM, Patel BB, Bahrami-Nejad Z, Travaglini KJ, Ahmed Y, Lee E, Carette JE^, and Rohatgi R^. (^corresponding author)
eLife 5: e21459 (2016). PMID: 27996937.

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cited by 66 [Google Scholar]

Cholesterol activates the G-protein coupled receptor Smoothened to promote Hedgehog signaling.

Luchetti G*, Sircar R*, Kong JH, Nachtergaele S, Sagner A, Byrne EF, Covey DF, Siebold C^, and Rohatgi R^. (*equal contribution; ^corresponding author)
eLife 5: e20304 (2016). PMID: 27705744.

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cited by 285 [Google Scholar]

Chromatin-remodeling complex SWI/SNF controls multidrug resistance by transcriptionally regulating the drug efflux pump ABCB1.

Dubey R*, Lebensohn AM*, Bahrami-Nejad Z, Marceau C, Champion M, Gevaert O, Sikic BI, Carette JE^, and Rohatgi R^. (*equal contribution; ^corresponding author)
Cancer Research 76(19):5810-5821 (2016). PMID: 27503929.

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cited by 51 [Google Scholar]

In vivo formation of vacuolated multi-phase compartments lacking membranes.

Schmidt HB^ and Rohatgi R^. (^corresponding author)
Cell Reports 16(5):1228-1236 (2016). PMID: 27452472.

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cited by 206 [Google Scholar]

Structural basis of Smoothened regulation by its extracellular domains.

Byrne EFX*, Sircar R*, Miller PS, Hedger G, Luchetti G, Nachtergaele S, Tully MD, Mydock-McGrane L, Covey DF, Rambo RP, Sansom MSP, Newstead S*, Rohatgi R^, and Siebold C^. (*equal contribution; ^corresponding author)
Nature 535(7613):517-522 (2016). PMID: 27437577.

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cited by 423 [Google Scholar]

An essential role for Grk2 in Hedgehog signaling downstream of Smoothened.

Zhao Z*, Lee RT*, Pusapati GV, Iyu A, Rohatgi R, and Ingham PW^. (*equal contribution; ^corresponding author)
EMBO Reports 17(5):739-752 (2016). PMID: 27113758.

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cited by 62 [Google Scholar]

2015

Functional Divergence in the Role of N-Linked Glycosylation in Smoothened Signaling.

Marada S, Navarro G, Truong A, Stewart DP, Arensdorf AM, Nachtergaele S, Angelats E, Opferman JT, Rohatgi R, McCormick PJ, and Ogden SK^. (^corresponding author)
PLoS Genetics 11(8):e1005473 (2015). PMID: 26291458.

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cited by 55 [Google Scholar]

Notch activity modulates the responsiveness of neural progenitors to Sonic Hedgehog signaling.

Kong JH*, Yang L*, Dessaud E, Chuang K, Moore DM, Rohatgi R, Briscoe J, Novitch BG. (*equal contribution)
Developmental Cell 33(4):373-387 (2015). PMID: 25936505

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cited by 164 [Google Scholar]

Measuring Expression Levels of Endogenous Gli Genes by Immunoblotting and Real-Time PCR.

Niewiadomski P, Rohatgi R^. (^corresponding author)
Natalia A. Riobo (ed.), Hedgehog Signaling Protocols, Methods in Molecular Biology 1322: 81-92 (2015). PMID: 26179041

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cited by 14 [Google Scholar]

Rapid Screening of Gli2/3 Mutants Using the Flp-In System.

Niewiadomski P, Rohatgi R^. (^corresponding author)
Natalia A. Riobo (ed.), Hedgehog Signaling Protocols, Methods in Molecular Biology 1322: 125-130 (2015). PMID: 26179044

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cited by 4 [Google Scholar]

Measuring Gli2 Phosphorylation by Selected Reaction Monitoring Mass Spectrometry.

Ahrends R*, Niewiadomski P*, Teruel MN, Rohatgi R^. (^corresponding author)
Natalia A. Riobo (ed.), Hedgehog Signaling Protocols, Methods in Molecular Biology 1322: 105-123 (2015). PMID: 26179043

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cited by 1 [Google Scholar]

2014

Location, location, and location: compartmentalization of Hedgehog signaling at primary cilia.

Pusapati GV^ and Rohatgi R^. (^corresponding author)
The EMBO Journal 33(17):1852-1854 (2014). PMID: 25037564.

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cited by 13 [Google Scholar]

Frontiers in hedgehog signal transduction.

Guerrero I^ and Rohatgi R^. (^corresponding author)
Seminars in Cell & Developmental Biology 33:50-51 (2014). PMID: 24946961.

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cited by 1 [Google Scholar]

A novel osteogenic oxysterol compound for therapeutic development to promote bone growth: activation hedgehog signaling and osteogenesis through smoothened binding.

Montgomery SR, Nargizyan T, Meliton V, Nachtergaele S, Rohatgi R, Stappenbeck F, Jung ME, Johnson JS, Aghdasi B, Tian H, Weintraub G, Inoue H, Atti E, Tetradis S, Pereira RC, Hokugo A, Alobaidaan R, Tan Y, Hahn TJ, Wang JC, and Parhami F^. (^corresponding author)
Journal of Bone and Mineral Research 29(8):1872-85 (2014). PMID: 24591126.

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cited by 88 [Google Scholar]

G-protein-coupled receptors, Hedgehog signaling and primary cilia.

Mukhopadhyay S^ and Rohatgi R^. (^corresponding author)
Seminars in Cell & Developmental Biology 33: 63-72 (2014). PMID: 24845016.

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cited by 176 [Google Scholar]

Tracking the subcellular fate of 20(S)-hydroxycholesterol with click chemistry reveals a transport pathway to the Golgi.

Peyrot SM*, Nachtergaele S*, Luchetti G, Mydock-McGrane LK, Fujiwara H, Scherrer D, Jallouk A, Schlesinger PH, Ory DS, Covey DF, and Rohatgi R^. (*equal contribution; ^corresponding author)
The Journal of Biological Chemistry 289(16):11095-11110 (2014). PMID: 24596093.

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cited by 27 [Google Scholar]

EFCAB7 and IQCE regulate Hedgehog signaling by tethering the EVC-EVC2 complex to the base of primary cilia.

Pusapati GV*, Hughes CE*, Dorn KV*, Zhang D, Sugianto P, Aravind L^, and Rohatgi R^. (*equal contribution; ^corresponding author)
Developmental Cell 28(5):483-496 (2014). PMID: 24582806.

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cited by 112 [Google Scholar]

Gli protein activity is controlled by multi-site phosphorylation in vertebrate Hedgehog signaling.

Niewiadomski P^, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel MN, Novitch BG, and Rohatgi R^. (^corresponding author)
Cell Reports 6(1):168-81 (2014). PMID: 24373970.

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cited by 315 [Google Scholar]

Isolation and mutational analysis of circulating tumor cells from lung cancer patients with magnetic sifters and biochips.

Earhart CM, Hughes CE, Gaster RS, Ooi CC, Wilson RJ, Zhou LY, Humke EW, Xu L, Wong DJ, Willingham SB, Schwartz EJ, Weissman IL, Jeffrey SS, Neal JW, Rohatgi R, Wakelee HA, and Wang SX^. (^corresponding author)
Lab on a Chip 14(1), 78-88 (2014). PMID: 23969419.

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cited by 212 [Google Scholar]

2013

Structure and function of the Smoothened extracellular domain in vertebrate Hedgehog signaling.

Nachtergaele S*, Whalen DM*, Mydock LK, Zhao Z, Malinauskas T, Krishnan K, Ingham PW, Covey DF, Siebold C^, and Rohatgi R^. (*equal contribution; ^corresponding author)
eLife 2:e01340 (2013). PMID: 24171105.

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cited by 205 [Google Scholar]

cited by 212 [Google Scholar]

Chemically inducible diffusion trap at cilia reveals molecular sieve-like barrier.

Lin YC, Niewiadomski P*, Lin B*, Nakamura H*, Phua SC, Jiao J, Levchenko A, Inoue T, Rohatgi R, and Inoue T^. (*equal contribution; ^corresponding author)
Nature Chemical Biology 9(7):437-443 (2013).  PMID: 23666116.

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cited by 159 [Google Scholar]

Cancer risk after use of recombinant bone morphogenetic protein-2 for spinal arthrodesis.

Carragee EJ, Chu G, Rohatgi R, Hurwitz EL, Weiner BK, Yoon ST, Comer G, and Kopjar B. 
Journal of Bone and Joint Surgery 95(17), 1537-45 (2013). PMID: 24005193.

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cited by 31 [Google Scholar]

2012

Oxysterols are allosteric activators of the oncoprotein Smoothened.

Nachtergaele S, Mydock LK, Krishnan K, Rammohan J, Schlesinger PH, Covey DF^, and Rohatgi R^. (^corresponding author)
Nature Chemical Biology 8(2):211-20 (2012). PMID: 22231273.

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A Smoothened-Evc2 complex transduces the Hedgehog signal at primary cilia.

Dorn K, Hughes CE and Rohatgi R^. (^corresponding author)
Developmental Cell 23(4):823-35 (2012). PMID: 22981989.

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cited by 222 [Google Scholar]

Singapore signalling: the 2012 hedgehog pathway cocktail.

Briscoe J^ and Rohatgi R^. (^corresponding author)
EMBO Reports 13(7):580-3 (2012). PMID: 22688966.

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2011

Cilia 2010: the surprise organelle of the decade.

Smith EF^ and Rohatgi R^. (^corresponding author)
Science Signaling 4(155):mr1 (2011). PMID: 21224442

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cited by 22 [Google Scholar]

2010

The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteins.

Humke EW, Dorn KV, Milenkovic L, Scott MP, and Rohatgi R^. (^corresponding author)
Genes & Development 24(7):670-82 (2010). PMID: 20360384.

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Role of lipid metabolism in smoothened derepression in hedgehog signaling.

Yavari A*, Nagaraj R*, Owusu-Ansah E, Folick A, Ngo K, Hillman T, Call G, Rohatgi R, Scott MP, and Banerjee U^. (*equal contribution; ^corresponding author)
Developmental Cell 19(1):54-65 (2010). PMID: 20643350

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The ciliary membrane.

Rohatgi R^ and Snell WJ^. (^corresponding author)
Current Opinion in Cell Biology 22(4):541-546 (2010). PMID: 20399632.

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cited by 249 [Google Scholar]

2009

Hedgehog signal transduction by Smoothened: pharmacologic evidence for a 2-step activation process.

Rohatgi R*, Milenkovic L*, Corcoran RB, and Scott MP^. (*equal contribution; ^corresponding author)
Proceedings of the National Academy of Sciences USA 106(9):3196-3201 (2009). PMID: 19218434.

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cited by 370 [Google Scholar]

Lateral transport of Smoothened from the plasma membrane to the membrane of the cilium.

Milenkovic L, Scott MP^, and Rohatgi R^. (^corresponding author)
The Journal of Cell Biology 187(3): 365-374 (2009). PMID: 19948480

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cited by 336 [Google Scholar]

2008

Arrestin' movement in cilia.

Rohatgi R and Scott MP^. (^corresponding author)
Science 320(5884): 1777-1781 (2008). PMID: 18583599

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2007

Patched1 regulates hedgehog signaling at the primary cilium.

Rohatgi R*, Milenkovic L*, and Scott MP^. (*equal contribution; ^corresponding author)
Science 317(5836): 372-376 (2007). PMID: 17641202

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Patching the gaps in Hedgehog signaling.

Rohatgi R and Scott MP^. (^corresponding author)
Nature Cell Biology 9(9): 1005-1009 (2007). PMID: 17762891

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cited by 264 [Google Scholar]

2006

In vitro reconstitution of cdc42-mediated actin assembly using purified components

Ho HY, Rohatgi R, Lebensohn A, and Kirschner MW. (^corresponding author)
Methods in Enzymology 406: 174-190 (2006). PMID: 16472658

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2004

Loss-of-function analysis of EphA receptors in retinotectal mapping.

Feldheim DA, Nakamoto M, Osterfield M, Gale NW, DeChiara TM, Rohatgi R, Yancopoulos GD, Flanagan JG^. (^corresponding author)
The Journal of Neuroscience 24(10): 2542-2550 (2004). PMID: 15014130

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cited by 183 [Google Scholar] 

Toca-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex.

Ho HY*, Rohatgi R*, Lebensohn A, Ma L, Li L, Gygi SP, and Kirschner MW*. (*equal contribution; ^corresponding author)
Cell 118(2): 203-216 (2004). PMID: 15260990

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cited by 550 [Google Scholar]

2002

Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck.

Eden S, Rohatgi R, Podtelejnikov AV, Mann M, and Kirschner MW^. (^corresponding author)
Nature 418(6899): 790-793 (2002). PMID: 12181570

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cited by 1167 [Google Scholar]

2001

CR16 forms a complex with N-WASP in brain and is a novel member of a conserved proline-rich actin-binding protein family.

Ho HY*, Rohatgi R*, Ma L, and Kirschner MW^. (*equal contribution; ^corresponding author)
Proceedings of the National Academy of Sciences USA 98(20): 11306-11311 (2001). PMID: 11553796

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Nck and phosphatidylinositol 4,5-bisphosphate synergistically activate actin polymerization through the N-WASP-Arp2/3 pathway.

Rohatgi R, Nollau P, Ho HY, Kirschner MW^, and Mayer BJ. (^corresponding author)
The Journal of Biological Chemistry 276(28): 26448-26452 (2001). PMID: 11340081

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cited by 540 [Google Scholar] 

WIP regulates N-WASP-mediated actin polymerization and filopodium formation.

Martinez-Quiles N, Rohatgi R, Anton IM, Medina M, Saville SP, Miki H, Yamaguchi H, Takenawa T, Hartwig JH, Geha RS^, and Ramesh N. (^corresponding author) 
Nature Cell Biology. 3(5): 484-491 (2001). PMID: 11331876

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cited by 370 [Google Scholar]

2000

Mechanism of N-WASP activation by CDC42 and phosphatidylinositol 4, 5-bisphosphate.

Rohatgi R*, Ho HY*, and Kirschner MW^. (*equal contribution; ^corresponding author)
The Journal of Cell Biology 150(6): 1299-1310 (2000). PMID: 10995436

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cited by 869 [Google Scholar]

1999

The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly.

Rohatgi R*, Ma L*, Miki H, Lopez M, Kirchhausen T, Takenawa T, and Kirschner MW^. (*equal contribution; ^corresponding author)
Cell 97(2): 221-231 (1999). PMID: 10219243

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cited by 1808 [Google Scholar]

1998

The Arp2/3 complex mediates actin polymerization induced by the small GTP-binding protein Cdc42.

Ma L, Rohatgi R, and Kirschner MW^. (^corresponding author)
Proceedings of the National Academy of Sciences USA. 95(26): 15362-15367 (1998). PMID: 9860974

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cited by 300 [Google Scholar]

1996

Nonenzymatic, template-directed ligation of oligoribonucleotides is highly regioselective for the formation of 3'-5' phosphodiester bonds.

Rohatgi R, Bartel DP, and Szostak JW^. (^corresponding author)
Journal of the American Chemical Society 118(14):3340-3344 (1996). PMID: 11539268

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cited by 165 [Google Scholar]

Kinetic and mechanistic analysis of nonenzymatic, template-directed oligoribonucleotide ligation.

Rohatgi R, Bartel DP, and Szostak JW^. (^corresponding author)
Journal of the American Chemical Society 118(14):3332-3339 (1996). PMID: 11539267

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cited by 160 [Google Scholar]